Contacts:
Supervisors:
Fabio Di Francesco
Title:
Electrochemical endonuclease based biosensor for pathogenic bacteria detection.
Abstract:
The selectivity and specificity provided by the use of biological recognition elements in the development of sensing technologies has attracted much interest in areas such as diagnostic medicine, food industry, environmental monitoring, among others. The use of electrochemical transduction techniques has a number of advantages for the development of biosensors, such as low-cost, simplicity, low detection limits, selectivity and potential for miniaturization. These features make techniques based on electrochemical transduction very attractive for the analysis in complex samples. Their coupling of this transduction system with enzymes such as the endonucleases offer the possibility of a simplified method that can be adapted for the sensitive and specific detection of genetic material from virus, bacteria and other microorganisms directly from the clinical sample. This would overcome the high cost and long processing times of traditional culturing or molecular techniques. The aim of this project is the development of an electrochemical biosensor based on the use of an endonuclease as a detection platform for bacterial DNA molecules in clinical samples.
Publications:
• B. Melai, P. Salvo, N. Calisi, L. Moni, A. Bonini, C. Paoletti, T. Lomonaco, V. Mollica, R. Fuoco, F. Di Francesco. A graphene oxide pH sensor for wound monitoring. 2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). Orlando, FL, USA. DOI: 10.1109/EMBC.2016.7591092
• Kirchhain, N. Poma, P. Salvo, L. Tedeschi, B. Melai, F. Vivaldi, A. Bonini, M. Franzini, L. Caponi, A. Tavanti, F. Di Francesco, Biosensors for measuring matrix metalloproteinases: An emerging research field, TrAC Trends Anal. Chem. 110 (2019) 35–50. doi:https://doi.org/10.1016/j.trac.2018.10.027.
Oral communications at congress:
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Poster communications at congress:
• A. Bonini, E. Herrera, F. Vivaldi, B. Melai, N. Poma, P. Salvo, A. Kirchhain, F. Di Francesco. Disposable pH biosensor for urea measurement. XXVII Congress of the Analytical Chemistry Division, 2018. Bologna (Italy), 16-20 September.
